2% Bis Solution from Bio-Rad

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Item2% Bis Solution
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1610142
Brand/TrademarkLSG LSD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell densityNature CommunicationsSeptember 16, 2023Julia Eckert, Benoît Ladoux, René-Marc Mège, Luca Giomi, Thomas Schmidtwere prepared from a stock solution of 40% acrylamide: A (1610140, Bio Rad) and 2% bis-acrylamide: B (1610142, Bio Rad) in PBS. The ratio of these components were mixed according to the PAA gel stiffness of 25.0

(2) Spatial transcriptomics for profiling the tropism of viral vectors in tissuesNature BiotechnologySeptember 1, 2023Min J. Jang, Gerard M. Coughlin, Cameron R. Jackson, Xinhong Chen, Miguel R. Chuapoco, Julia L. Vendemiatti, Alexander Z. Wang, Viviana GradinaruN-hydroxysuccinimide ester (AA-NHS, A8060, MilliporeSigma), acrylamide (1610140, Bio-Rad), bisacrylamide (1610142, Bio-Rad), tetramethylethylenediamine (TEMED, T7024, MilliporeSigma), ammonium persulfate (APS, A3678,

(3) Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structuresJournal of Biological ChemistryAugust 1, 2023Robert Bucki, Daniel V. Iwamoto, Xuechen Shi, Katherine E. Kerr, Fitzroy J. Byfield, Łukasz Suprewicz, Karol Skłodowski, Julian Sutaria, Paweł Misiak, Agnieszka Z. Wilczewska, et al.(3-aminopropyl)trimethoxysilane (281778), and 70% (v/v) glutaraldehyde (G7776) were from Sigma-Aldrich. 2% (w/v) bis acrylamide (1610142) and 40% (w/v) acrylamide solutions (1610140) were from Bio-Rad Laboratories. TEMED (TB0508) was from

(4) Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samplesbioRxiv : the preprint server for biologyJuly 18, 2023Chen Y, Chauhan S, Gong C, Dayton H, Xu C, De La Cruz ED, Datta MS, Leong KW, Dietrich LEP, Tomer R.hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#1610140, BioRad), 0.05% w/v bisacrylamide (#1610142, BioRad), 4% paraformaldehyde (15710-S, Electron Microscopy Sciences), 1xPBS, deionized water, and 0.25%

(5) The Effect of Substrate Stiffness on Elastic Force Transmission in the Epithelial Monolayers over Short TimescalesCellular and Molecular BioengineeringJuly 13, 2023Aapo Tervonen, Sanna Korpela, Soile Nymark, Jari Hyttinen, Teemu O. Ihalainen(AA, stock 40%, #1610140, Bio-Rad Laboratories, Hercules, USA) and bis-acrylamide (Bis, stock 2%, #1610142, Bio-Rad Laboratories, Hercules, USA) with PBS in 15 ml falcon tube [64]. The following mixing ratios

(6) Nuclear lamina strain states revealed by intermolecular force biosensorNature CommunicationsJune 30, 2023Brooke E. Danielsson, Bobin George Abraham, Elina Mäntylä, Jolene I. Cabe, Carl R. Mayer, Anna Rekonen, Frans Ek, Daniel E. Conway, Teemu O. Ihalainen(mixing ratio 12.5% acrylamide (#1610140, Bio-Rad Laboratories, Hercules, USA), 7.5% Bis-acrylamide (#1610142, Bio-Rad Laboratories, Hercules, USA). The polymerization of the gel was initiated by adding TEMED (final

(7) Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generationeLifeJune 30, 2023Michael T Kimble, Matthew J Johnson, Mattie R Nester, Lorraine S Symingtoncompound, drug",Nocodazole,AbMole,Cat# M3194, "chemical compound, drug",2% Bis Solution,Bio-Rad,Cat# 1610142, "chemical compound, drug",40% Acrylamide Solution,Bio-Rad,Cat# 1610140, "chemical compound, drug","N,N,N’,N’-Tetramethyl-ethylenediamine

(8) Directionality of developing skeletal muscles is set by mechanical forcesNature CommunicationsMay 27, 2023Kazunori Sunadome, Alek G. Erickson, Delf Kah, Ben Fabry, Csaba Adori, Polina Kameneva, Louis Faure, Shigeaki Kanatani, Marketa Kaucka, Ivar Dehnisch Ellström, et al.containing HEPES 10 mM, 300 μl (75 μl) acrylamide 40% (1610140, Bio-Rad), 125 μl (30 μl) bisacrylamide 2% (1610142, Bio-Rad), 5 μl (5 μl) 10% ammonium persulfate diluted in water (A3678, Sigma), 0.5 μl (0.5 μl) N, N,

(9) Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENACell reportsApril 25, 2023Wang W, Taufalele PV, Millet M, Homsy K, Smart K, Berestesky ED, Schunk CT, Rowe MM, Bordeleau F, Reinhart-King CA.ammonium persulfate (APS),Biorad,1610700EDU, Acrylamide [40% (w/v) solution],Biorad,1610140, bis-acrylamide [2% (w/v) solution],Biorad,1610142, CSF-1,R&D System,216-MC, Ribose,Thermo Fisher Scientific,132365000, β-Mercaptoethanol,MilliporeSigma,444203, TRIZOL,Invitrogen,15596026, iQ SYBR Green Supermix

(10) Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cellsiScienceApril 21, 2023Lopez-Cavestany M, Hahn SB, Hope JM, Reckhorn NT, Greenlee JD, Schwager SC, VanderBurgh JA, Reinhart-King CA, King MR.acrylamide solution (1610140, BIO RAD, Hercules, CA, USA) to 0.175% (v/v) bis-acrylamide solution (1610142, BIO RAD, Hercules, CA, USA),.55 The 60kPa gel precursor solution was prepared with a ratio of 13.5%

(11) Nuclear export inhibition jumbles epithelial-mesenchymal states and gives rise to migratory disorder in healthy epitheliaeLifeFebruary 21, 2023Krull CM, Li H, Pathak A.,40% Acrylamide Solution,BIORAD,Cat. #:1610140,N/A "Chemical compound, drug",2% Bis Solution,BIORAD,Cat. #: 1610142,N/A "Chemical compound, drug",Bind silane,GE Healthcare Life Sciences,Cat. #: 17-1330-01,N/A "Chemical

(12) Full-length huntingtin is palmitoylated at multiple sites and post-translationally myristoylated following caspase-cleavageFrontiers in PhysiologyJanuary 13, 2023Fanny L. Lemarié, Shaun S. Sanders, Yen Nguyen, Dale D. O. Martin, Michael R. HaydenWestern Blot Analysis,Tween 20,Thermo Fisher Scientific,#BP337-500 Western Blot Analysis,2% Bis Solution,Bio-Rad,#1610142 Western Blot Analysis,"30% Acrylamide/Bis Solution, 29:1",Bio-Rad,#1610156 Western Blot Analysis,40% Acrylamide Solution

(13) A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistrySTAR ProtocolsDecember 16, 2022Zhengyuan Pang, Li YeCAS#28718-90-3 40% acrylamide solution,Bio-Rad,Cat#1610140 2% bis-acrylamide solution,Bio-Rad,Cat#1610142 32% PFA solution,Electron Microscopy Sciences,Cat#15714-S "Phosphate buffer saline (PBS), 10×, pH

(14) Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infectionCell reportsDecember 13, 2022Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM.(4% paraformaldehyde (EMS #15710), 1% acrylamide (Bio-Rad #1610140), 0.05% bis acrylamide (Bio-Rad #1610142), 0.0025 g/mL radical initiator (Wako Chemical #VA-044) in 1× final concentration PBS (Corning #46013CM)

(15) Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imagingNature CommunicationsDecember 1, 2022Yuechuan Lin, Nichaluk Leartprapun, Justin C. Luo, Steven G. Adie(Bio-Rad, 40% Acrylamide Solution, 1610140), bis-acrylamide crosslinker (Bio-Rad, 2% Bis Solution, 1610142), and deionized water were mixed at appropriate concentrations (see Table 1 in ref. 37. for the compositions

(16) Cooperation between myofibril growth and costamere maturation in human cardiomyocytesFront Bioeng BiotechnolNovember 1, 2022Huaiyu Shi, Chenyan Wang, Bruce Z. Gao, James H. Henderson, Zhen Mapreparation of prepolymer solution, made of acrylamide (BIO-RAD Ca# 1610140), bis-acrylamide (BIO-RAD Ca# 1610142), 35 mM HEPES buffer (SIGMA Ca# 7365-45-9), 0.1% w/v ammonium persulfate (BIO-RAD Ca# 1610700), 0.1%

(17) In situ identification of cellular drug targets in mammalian tissueCellMay 12, 2022Pang Z, Schafroth MA, Ogasawara D, Wang Y, Nudell V, Lal NK, Yang D, Wang K, Herbst DM, Ha J, et al.Laemmli Sample Buffer,Bio-Rad,Cat#1610747 40% acrylamide solution,Bio-Rad,Cat#1610140 2% Bis Solution,Bio-Rad,Cat#1610142 EMS 32% Paraformaldehyde Aqueous Solution,Electron Microscopy Sciences,Cat#15714-S VA-044,Fisher Scientific,Cat#NC0632395;

(18) Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomographySTAR ProtocolsMarch 1, 2022Vaibhao Janbandhu, Ella M.M.A. Martin, Gavin Chapman, Sally L. Dunwoodie, Richard P. HarveyL6146 40% Acrylamide,Bio-Rad Laboratories,Cat# 1610140 2% Bis-acrylamide,Bio-Rad Laboratories,Cat# 1610142 VA044,NOVA Chemicals,Cat# 017-19362 Saponin,Sigma-Aldrich,Cat# S4521 Paraformaldehyde (PFA),Sigma-Aldrich,Cat#

(19) Early construction of the thalamocortical axon pathway requires c‐Jun N‐terminal kinase signaling within the ventral forebrainDevelopmental DynamicsMarch 1, 2022Jessica G. Cunningham, James D. Scripter, Stephany A. Nti, Eric S. Tuckerw/v paraformaldehyde (pH 7.2), 4% w/v acrylamide (Bio-Rad 1610140), 0.05% w/v bis-acrylamide (Bio-Rad 1610142), 0.25% w/v VA044 initiator (Fisher Scientific, NC0632395), 0.05% w/v Saponin (EMD Millipore, 558255),

(20) Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarctionCell stem cellFebruary 3, 2022Janbandhu V, Tallapragada V, Patrick R, Li Y, Abeygunawardena D, Humphreys DT, Martin EMMA, Ward AO, Contreras O, Farbehi N, et al.solution,Sigma-Aldrich,Cat# L6146 40% Acrylamide,Bio-Rad,Cat# 1610140 2% Bis-acrylamide,Bio-Rad,Cat# 1610142 Saponin,Sigma-Aldrich,Cat# S4521 PhosSTOP,Sigma-Aldrich,Cat# 4906845001 cOmplete Protease Inhibitor

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2% Bis Solution

2% Bis Solution from Bio-Rad

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2% Bis Solution from Bio-Rad

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